Optical clearing of the mouse brain and light attenuation quantitation
نویسندگان
چکیده
Optical clearing allows the reduction of light scattering in biological tissue, enabling 3D morphological information to be obtained deep within tissue using techniques such as optical projection tomography and light sheet microscopy. However, the extent of the clearing is dependent on the technique that is used. There is therefore a need for methods to quantify the quality of the clearing process and thereby to compare clearing techniques. In this study, we developed such a method using a custom spectroscopy system and applied it to compare three techniques that were applied to mouse brain: BABB (Murray’s clear), pBABB (a modification of BABB which includes the use of hydrogen peroxide), and passive CLARITY.
منابع مشابه
Quantification of light attenuation in optically cleared mouse brains.
Optical clearing, in combination with recently developed optical imaging techniques, enables visualization and acquisition of high-resolution, three-dimensional images of biological structures deep within the tissue. Many different approaches can be used to reduce light absorption and scattering within the tissue, but there is a paucity of research on the quantification of clearing efficacy. Wi...
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